Analysis of viral DNA associated with H5pm8001 intermediates. Viral DNA was prepared from purified particles, digested with KpnI and ClaI, and analyzed by Southern with an Ad5-specific probe. Lanes: pm8001, intermediates from H5pm8001-infected 293 cells; MV and EC, mature virions and empty capsids, respectively, prepared from Ad5-infected 293 cells; ts369, particles prepared from 293 cells infected with H5ts369 at 39.5°C. (B) Longer exposure of the blot shown in panel A, with lane 1 removed. The arrow indicates the left end-specific ClaI fragment.