FIG. 6.

Expression of viral proteins in H5pm8001-infected 293 cells. Twenty-five micrograms of whole-cell lysates prepared from 293 cells infected with H5pm8001 or Ad5 at the indicated times postinfection were analyzed by immunoblot using antibodies to the 52/55-kDa protein (52/55K) (A), to the E2A 72-kDa DNA binding protein (72K) (B), to the IVa2 protein (C), and to the fiber protein (D). hpi, hours postinfection. The faster-migrating band in panel B is a degradation product of the 72K protein (54). The locations of molecular weight markers are indicated on the left.