FIG. 9.

Analysis of the protein composition of H5pm8001 intermediates. Particles were purified by two rounds of CsCl gradient centrifugation and analyzed by silver staining. Lanes: pm8001, intermediates from H5pm8001-infected 293 cells; MV and EC, mature virions and empty capsids, respectively, prepared from Ad5-infected 293 cells; ts369, particles prepared from H5ts369-infected 293 cells at 39.5°C. The identities of several components of the mature virion are indicated based on their mobility in the gel. The arrow denotes the 70-kDa protein described in the text and molecular weight markers are indicated on the left.