Figure 1.

Effect of culture medium on morphology and proliferation of C-MSC. Cells were cultured in M199 (A-E) or SCM (F-J). (A, F) Representative phase contrast images at day 3 of culture (scale bar = 90 μm). (B, G) Representative phase contrast images at day 7 of culture (scale bar = 90 μm). (C, H) Representative images of vimentin expression (red) counterstained with phalloidin (F-actin, green) and DAPI (blue, scale bar=100 μm). (D, I) Representative images of Pan-Cytokeratin staining (green) counterstained with DAPI (blue, scale bar=100 μm). (E, J) Relative viability of C-MSC cultured 17 days (RFU, relative fluorescence units). Data shown as mean ± SEM of three independent experiments with different C-MSC donors (n = 3) each with six replicates.