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. 2024 Apr 11;33:09636897241241992. doi: 10.1177/09636897241241992

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© The Author(s) 2024

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Comparative effect of culture medium on protein expression of C-MSC at P1 and P4. C-MSC were continually cultured in (a, b) M199 or (c, d) SCM and immunocytochemistry was performed at passage 1 (P1; a, c) and passage 4 (P4; b, d) for (A) CD34 (green), (B) CD105 (red) shown merged with CD34 (green), (C) CD90 (green), (D) CD73 (red) shown merged with CD90 (green), I ABCG2 (green), (F) SSEA-4 (green), (G) Oct4A (red), (H) α-SMA (red) shown merged with ALDH3A1 (green), and (I) keratocan (green). Representative images shown of three independent experiments with three different C-MSC donors (n = 3) with DAPI counterstain (blue), scale bar=100 μm.