Figure 6.

Effect of siRNA-mediated knockdown of CD34 on C-MSC gene expression. C-MSC cultured in either M199 or SCM were transfected with either non-targeting siRNA or CD34 siRNA at passage 2. Relative levels of mRNA following transfection were determined by RT-qPCR for the following genes: (A) CD34 (B) ABCG2, (C) PAX6, (D) POU5F1, (E) NANOG, (F) REX1, (G) SOX2, (H) NT5E, (I) THY1, (J) ENG. Expression of each target gene was normalized to GAPDH and represented relative to the mRNA expression of untreated cells in the corresponding media. Data shown as mean ± SEM of three independent experiments with three different C-MSC donors (n = 3), each with two replicates. Statistical significance of knockdown vs. untreated cells: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.