Figure 7.

Potential anti-inflammatory effect of C-MSC cultured in SCM. (A-E) C-MSC cultured in M199 or SCM were exposed to 1 µg/mL LPS for 72 h. (A) Relative cell viability at 72 h. Data shown relative to control of same media. (B) Cytotoxic effect of LPS (LDH production). (C) Nitrite accumulation in the culture medium. (D) IL-6 production. (E) IL-8 accumulation in the culture medium. Data corrected for relative cell viability. B-E corrected for relative cell viability. Data shown as mean ± SEM of three independent experiment (n = 3) each with two replicates. Statistical significance of control vs. LPS: *P ≤ 0.05, ****P ≤ 0.0001. Statistical significance of M199 vs. SCM: ##P ≤ 0.01, ####P ≤ 0.0001. (F-I). Response of ihCEC injury model to co-culture with C-MSC. ihCEC treated with 20% ethanol for 30 s, followed by exposure to 1 µg/mL LPS. C-MSC, M199 or SCM cultured, were co-cultured with the ihCEC immediately after ethanol injury, during LPS stimulation. (F) Relative cell viability at 72 h. Data shown relative to no co-culture control. (G) Cytotoxic effect on ihCEC measured by LDH production. Data corrected for production of LDH by C-MSC-only controls and relative cell viability. (H) IL-6 accumulation in the culture medium. (D) IL-8 accumulation in the culture medium. G, H, I corrected for production by C-MSC-only controls and relative cell viability. All data shown as mean ± SEM of three independent experiment with three different C-MSC donors (n = 3) each with two replicates. Statistical significance of control vs. injury: *P ≤ 0.0001. Statistical significance vs. no co-culture control: #P ≤ 0.0001. Statistical significance vs. no co-culture injury: +P ≤ 0.0001.