Skip to main content
. 2024 Mar 22;5:100174. doi: 10.1016/j.crpvbd.2024.100174

Fig. 2.

Fig. 2

Agarose gel image of the multiplex molecular xenomonitoring PCR during sensitivity (analytical limit-of-detection) testing. Abbreviations: HL, HyperLadder 100 bp (BioLine, UK); Sm, Schistosoma mansoni sample DNA; Sr, S. rodhaini sample DNA; NTC, no template ddH2O negative control; Bp, Biomphalaria pfeifferi sample DNA. Whilst the strength of the Biomphalaria ITS agarose gel band remained constant in all reactions, as S. mansoni and S. rodhaini DNA concentration decreased, the strength of the Schistosoma ITS gel bands (both S. mansoni and S. rodhaini) and the S. mansoni ND5 gel band decreased. The multiplex assay proved capable of detecting S. mansoni DNA with a limit-of-detection of between 0.2 ng and 0.02 ng and proved capable of detecting S. rodhaini DNA with a limit-of-detection of between 2 ng and 0.2 ng. GelRed loading buffer stain may affect PCR amplicon migration.