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. 2024 Mar 22;5:100174. doi: 10.1016/j.crpvbd.2024.100174

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© 2024 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Agarose gel image of the high throughput multiplex molecular xenomonitoring PCR assay during pilot application. Abbreviations: HL, HyperLadder 100 bp (BioLine, UK); NTC, no template ddH₂O negative control; Bp, Biomphalaria pfeifferi sample DNA; Sm, Schistosoma mansoni sample DNA; Sr, S. rodhaini sample DNA. A: Bp+Sm(mal) sample infected with S. mansoni (detected during cercarial shedding); B and C: Two Biomph91(mal) samples infected with S. mansoni (not detected during cercarial shedding); D and E: Two Biomph91(mal) samples infected with Uvulifer spp. All remaining samples are Biomph91(mal) samples not found to be infected with any trematode species, including S. mansoni. GelRed loading buffer stain may affect PCR amplicon migration.