Figure 13.

Low expression of Cathepsin D promotes the acquisition of an epithelial phenotype.Sham, KD-CD and Over CD clones were seeded in 3D cultures and allowed to grow for 4 days. Later, 3D spheroids were transferred on to poly-lysine-coated coverslips and allowed to grow for a further 2 days. At the end of the experiment, coverslips were fixed and stained for immunofluorescence double staining of CD (red)–E-cadherin (green) or CD (red)–N-cadherin (green). Nuclei were stained with DAPI. Scale bar = 20 µm; magnification = 63×. Representative images of three independent experiments are shown.