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. 2024 Mar 27;25(7):3715. doi: 10.3390/ijms25073715

Figure 4.

Figure 4

Treatment with CAEE significantly reduces DNA damage. (A) Representative images illustrating DNA damage assessed by staining with anti-53BP1 antibody (green) in WJ-MSCs treated with CAEE or not (NT) where nuclei were visualised using DAPI (blue). Images were acquired using fluorescence microscope (40× objective lens). (B) Bar chart depicts the percentage of cells with DNA damage (cells with 4 or more 53BP1 foci) per condition (treated with CAEE or NT). The experiment was repeated three times with cells from 3 different donors, and each time at least 5 different fields were examined for each condition, measuring up to at least 100 different cells. The values depicted are the means ± S.E. Asterisk marks statistical significance (p < 0.05) in all panels (* = p < 0.05).