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. 2024 Mar 27;25(7):3715. doi: 10.3390/ijms25073715

Figure 7.

Figure 7

CAEE treatment stimulates autophagy to counteract SIPS induction. (A) Representative immunoblots of autophagy-related proteins in WJ-MSCs treated with 400 μΜ H2O2 for 2 h to induce premature senescence at passage 2 (SIPS p2) and WJ-MSCs treated with CAEE (for 24 h) prior to senescence induction (CAEE+SIPS p2). (B,C) Representative immunoblots of p62 and ULK-1 proteins in WJ-MSCs treated with 400 μΜ H2O2 for 2 h to induce premature senescence at passage 2 (SIPS p2) and WJ-MSCs treated with CAEE (for 24 h) prior to senescence induction (CAEE+SIPS p2). Densitometric analysis of ULK-1 (D), PI3K-CIII (E), ATG5 (F), BECLIN-1 (G), p62 (H), and LC3II/I (I) protein levels (fold change) in WJ-MSCs SIPS p2 and WJ-MSCs CAEE+SIPS p2. SIPS p2 value was arbitrarily set to 1. The values depicted are the means ± S.E. from three different experiments with cells from three different donors (n = 3). Asterisk marks statistical significance (p < 0.05) in all panels (* = p < 0.05, ** = p < 0.01).