Table 5.
Composition, mode of delivery, and dosage schedules of selected synthetic inhibitors or mimics of microRNAs.
| Synthetic Molecule ϕ Ref. | Organism | Composition | MoD | Dosage Schedules |
|---|---|---|---|---|
| AntimiRS | ||||
| LNA-antimiR-29 [17] | Mouse | Saline | I.V | 20 mg/kg, 1 daily dose for 3 days, starting d1 after surgery. |
| LNA-antimiR-15b [188] | Mouse | Saline | i.v. via catheter | Up to 33 mg/kg, 1 dose 3 days after AngII infusion. |
| LNA-antimiR-26a or miR-26a mimic [189] | Mouse | Matrigel | s.c | 1 × 106 cells/mL Matrigel transfection: 30–100 nM oligonucleotide/5 × 104 cells |
| LNA-antimiR-15 [190] | Mouse | Saline | s.c | 2 doses with 5 mg/kg each (2–3 days before TAC, 3–4 days after) |
| LNA-antimiR-26a [190] | Mouse | Not candidate | i.v | 24 mg/kg, 1 dose 24 h before MI |
| LNA-antimiR-15b [188] | Pig | Saline | i.v | Up to 3.3 mg/kg |
| LNA-antimiR-22 [191] | Mouse | Hydrogel | Perivascular | 2.5 nmol Injection concomitant with surgery |
| LNA-antimiR-21 [18] | Pig | Saline | i.v | 10 mg each on d5 and d19 after MI |
| Antagomirs | ||||
| Antagomir-199b [38] | Mouse | Saline | i.p | 0.05–80 mg/kg |
| Antagomir-25 [192] | Mouse | Saline | i.p | 80 mg/kg, 1 daily dose for 3 days, starting day 1 after surgery |
| Antagomir-21 [18] | Mouse | Saline | i.v. via catheter | 80 mg/kg, 1 daily dose for 2 days, starting d1 or d21 after surgery. |
| Antagomir-29b [117] | Mouse | Saline | i.p | 80 mg/kg, 1 daily dose for 2 days, starting d1 or d21 after surgery |
| Antagomir-146a [193] | Mouse | Saline | Not indicated | 8 mg/kg d2 before delivery and d1, d3, and d7 after surgery |
Table 5 displays the therapeutic dosage and composition administered in the animal model. Abbreviations used in this text include the following: MoD, mode of delivery; AngII, angiotensin II; i.v., intravenous; s.c., subcutaneous; i.p., intraperitoneal.