FIG. 7.
Effect of IκBα S32AS36A overexpression on LMP1-mediated Fas up-regulation. BL41 cells were transfected with 3 μg of GFP expression vector and the indicated amounts (in micrograms) of pSG5 LMP1 and pCMV4 IκBα S32AS36A. Total DNA transfected was kept constant by addition of vector DNA. (A) Approximately 20 h posttransfection, one fraction of the cells was tested for Fas, ICAM-1, CD40, and LFA-3 surface expression by two-color FACS analysis as described for Fig. 6. Only data for Fas are shown. (B) Whole-cell lysates (5 × 105 cells per lane) were analyzed by immunoblotting for LMP1 expression with anti-LMP1 monoclonal antibody S12.