Figure 5.

The PARPi PJ34 and the PARP14i MCD113 decreased M2 marker gene expression in JIMT-1-THP-1-derived MΦ co-culture spheroids. Spheroids were grown from co-cultures of JIMT-1 cells and THP-1-derived MΦs or from THP-1-derived MΦs only, as described in Section 4. Spheroids were kept in cultures for 4 days after formation (1 day in the presence of 20 ng/mL IFN-γ), followed by treatments with either 25 μM PJ34 (A) or 55 μM MCD113 (B) for 24 h. Columns show the relative expression of mRNA levels of M2 markers in spheroids and spheroid co-cultures quantified by RT-qPCR. The histograms represent the mean of at least 3 independent experiments (±SEM). Statistical evaluation was performed with one-way ANOVA and Tukey’s post-hoc test (MRC1 and CCL17 in (A), Fibronectin in (A,B)), or with the Kruskal–Wallis test and Dunn’s post-hoc test (* p < 0.05, ns: not significant).