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. 2024 Mar 28;13(7):590. doi: 10.3390/cells13070590

Figure 4.

Figure 4

Spontaneously reversible osmosensing by GFP-MxA condensates in oral epithelial cells-focus on saliva-like hypotonicity. (A) Sequential live-cell imaging of the same OECM1 cell expressing GFP-MxA condensates 2 days after transient transfection first in full-culture medium (330 mOsm) and then after shifting to hypotonic of one-third tonicity (110 mOsm; full medium diluted 1:2 with water) for the next 8–10 min. Scale bar = 10 µm. (B) Quantitation of GFP-MxA in condensates on a % per-cell basis in the images shown in (A). This quantitation was carried out using the small object subtract Filter in Image J [20,24].