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Testing a biochemical basis for hypotonicity sensing by GFP-MxA condensates in oral epithelial cells. (A,B), OECM1 cells were either exposed to CsA (25 µM) or DMSO alone for 20 min in full-culture medium, and then shifted to one-third tonicity medium in the continued presence of CsA. Live-cell imaging was carried out as indicated. Scale bar = 10 µm. (C) Quantitation of % GFP-MxA per cell in condensates (in the same cells shown in (A,B)).
