FIG. 4.

sCAR protein inhibits binding to Ramos cells. Equal specific activity input doses (9,000 cpm) of radiolabeled Ad serotypes were incubated with sCAR protein at 10 μg/ml for 30 min at RT. Next, the virus was added to 2 × 10⁶ Ramos cells and incubated in suspension for 1 h at RT. The cells were washed, the pellet was resuspended in 100 μl of PBS, and bound virus was determined by scintillation counting. The bars represent cpm bound (mean of three assays ± 5%). CTRL is control binding without sCAR preincubation.