FIG. 5.

Qualitative analysis of Ad binding to CHO-CAR cells. Approximately 10⁶ CHO-CAR cells, or CHO-CAR cells preincubated with F5K protein at 5 μg/ml, were incubated for 1 h at 4°C with radiolabeled Ad serotypes. The cells were washed, the pellet was resuspended in 100 μl of PBS, and bound virus was determined by scintillation counting. Open bars represent binding of the viruses to CHO cells standardized to 1 (mean of 3 assays ± 6%). Hatched bars represent binding to CHO-CAR cells (fold increase over binding to CHO). Solid bars represent binding to CHO-CAR cells after preincubation with F5K protein (fold increase over binding to CHO). The counts measured (in cpm) were as follows: Ad12, 323, 5,021, 865; Ad2, 944, 10,299, 1,500; Ad9, 50, 320, 210; Ad4, 293, 4,772, 1,135; Ad41, 255, 8,567, 1,158.