FIG. 2.

Yeast two-hybrid assay to localize the interaction domain between Rep78 and PRKX. (a) pGBT-Rep78 contains the full-length Rep78 ORF for amino acids 1 to 621. In pGBT-Rep68 the intron from amino acid 529 is spliced out and the sequence LARGHSL occurs at the splice acceptor site (light grey). An EcoRI fragment extending from the pGBT cloning site to AAV nt 1767 was deleted to produce pGBT-Cterm, which encodes Rep amino acids 483 to 621. (b) Strain SFY56 yeast cells were transformed with pGBT and pGAD vectors in the combinations indicated on the x axis. β-Galactosidase activity was then measured by ONPG assay. Error bars indicate standard deviations.