Table 1.

HSA-NIT conjugate secondary structure.

Sample	NIT Residue Per HSA Molecule	CD Spectral Analysis
		α-Helix, %	β-Sheet, %
Monomer HSA	-	55.0 ± 1.0	4.8 ± 1.0
HSA-Me	13.7 ± 0.7	49.2 ± 1.5	5.9 ± 0.6
HSA-Me-mal	16.4 ± 0.9	44.3 ± 1.7	8.1 ± 0.6
HSA-Me/Me-mal	17.1 ± 1.0	43.7 ± 2.0	7.8 ± 0.8
HSA-Et	13.1 ± 0.2	48.8 ± 1.4	6.4 ± 0.6
HSA-Et-mal	21.9 ± 1.0	44.1 ± 1.7	10.9 ± 0.7
HSA-Et/Et-mal	20.8 ± 1.1	43.5 ± 2.0	9.7 ± 0.8