Table 1.

Polyphenolic content, expressed in mg/g dried olive leaf using different sample–solvent proportions.

Bioactive Compounds	S1	S2	S3	S4	S5	S6	S7	S8	S9
Hydroxytyrosol	0.72 ± 0.05 ab	0.77 ± 0.02 a	0.67 ± 0.05 ab	0.61 ± 0.07 b	0.49 ± 0.04 c	0.58 ± 0.06 bc	0.5 ± 0.05 c	0.44 ± 0.01 c	0.48 ± 0.07 c
P-coumaric acid	0.028 ± 0.003 a	0.032 ± 0.002 a	0.029 ± 0.002 ad	0.03 ± 0.01 ab	0.027 ± 0.001 bc	0.026 ± 0.001 bd	0.0218 ± 0.0005 a	0.03 ± 0.001 c	0.036 ± 0.002 e
Ferulic acid	0.011 ± 0.001 a	0.01 ± 0.002 a	0.0066 ± 0.0002 b	0.006 ± 0.001 b	0.0067 ± 0.0005 b	0.0062 ± 0.0002 b	0.009 ± 0.001 ac	0.007 ± 0.002 bc	0.0075 ± 0.0005 b
Chlorogenic acid	0.09 ± 0.01 ab	0.102 ± 0.003 a	0.06 ± 0.01 c	0.08 ± 0.01 b	0.06 ± 0.01 c	0.08 ± 0.01 b	0.031 ± 0.004 d	0.047 ± 0.003 cd	0.05 ± 0.01 c
Oleuropein	113 ± 5 a	123 ± 6 a	97 ± 3 b	81 ± 7 c	81 ± 7 c	85 ± 10 c	62 ± 6 d	69 ± 1 cd	88 ± 13 bc
Diosmin	0.09 ± 0.01 ab	0.11 ± 0.01 a	0.102 ± 0.004 ab	0.07 ± 0.01 c	0.08 ± 0.01 c	0.09 ± 0.01 b	0.06 ± 0.01 c	0.063 ± 0.003 c	0.09 ± 0.02 ab
Rutin	0.56 ± 0.03 ab	0.69 ± 0.03 c	0.57 ± 0.03 a	0.44 ± 0.05 bc	0.45 ± 0.04 abc	0.52 ± 0.07 ab	0.38 ± 0.05 c	0.4 ± 0.01 c	0.5 ± 0.08 abc
Verbascoside	4.4 ± 0.3 ab	5.3 ± 0.2 c	4.5 ± 0.2 ab	3.8 ± 0.5 bd	3.7 ± 0.3 bd	4.4 ± 0.5 ab	2.9 ± 0.3 d	3.37 ± 0.09 d	5.2 ± 0.3 a

S1: 2 g/100 mL, no storage time; S2: 5 g/100 mL, no storage time; S3: 12.5 g/100 mL, no storage time; S4: 2 g/100 mL, 1 month storage; S5: 5 g/100 mL, 1 month storage; S6: 12.5 g/100 mL, 1 month storage; S7: 2 g/100 mL, 2 months storage; S8: 5 g/100 mL, 2 months storage; S9: 12.5 g/100 mL, 2 months storage. Different letters among the same row indicate the presence of statistically significant differences.