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. 2024 Apr 4;25(7):4031. doi: 10.3390/ijms25074031

Figure 4.

Figure 4

Navitoclax and the combination of dasatinib/quercetin differentially regulate IL-6 expression and secretion levels. (A) The expression of IL-6 in CAF cells was evaluated using qPCR. Charts represent relative expression levels as mean values ± SEM normalized by Gapdh. (B) The IL-6 concentration in supernatants was measured by ELISA. Data are presented as mean ± SD (n = 3 replicates per group); data were analyzed using one-way ANOVA with Tukey’s multiple comparisons test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Control, N, D+Q - different shades of blue, TNF, N+TNF, D+Q+TNF - different shades of green. Control—CAFs incubated in DMEM/FBS for 5 days where replicative senescence gradually increased; N—CAFs incubated in DMEM/FBS supplemented with 0.5 µM navitoclax for 5 days; D + Q—CAFs incubated in DMEM/FBS supplemented with 0.1 µM dasatinib and 1 µM quercetin for 5 days; TNF—CAFs incubated in DMEM/FBS supplemented with 10 ng/mL TNF for 5 days.