Figure 1.

(A) Recombinant human lfTSLP (5 μg) was treated with recombinant human tryptase (0.5 μg) at 37 °C in the presence of heparin (1:10). Samples were withdrawn at 0, 30, 60, 120, and 240 min, and inactivated by heating for 10 min at 99 °C to stop the cleavage reaction. Each digestion mixture corresponding to 1 μg of protein was separated on 16% Tris-Tricine gel. The gel was stained with a colloidal Coomassie Brilliant Blue solution. (B) The reduction of the band intensity at ~15 kDa was quantified by densitometric analysis. The results show the mean ± SD of three independent experiments.