FIG. 6.

CTLA4Ig treatment does not reduce defective Gag mRNA expression. cDNA obtained from reaction of RNA samples of individual mice with RT were amplified by either defective-Gag- or HPRT-specific primers for 30 cycles. The bands for the two products appeared on a 2% agarose gel at the expected migration points, corresponding to 237 and 163 bp, respectively. Lanes: 2 and 3, samples from untreated, infected mice (SP weights, 450 and 477 mg); 4 and 5, samples from CTLA4Ig-treated, infected mice (SP weights, 165 and 199 mg); 1, negative control for PCR (water); 6, specificity control for PCR using a J1 plasmid containing the defective Du5H sequence. No amplification was obtained after PCR of RNA samples (data not shown). These results are representative of two to five mice per group.