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trans-encapsidation assay. HeLa cell monolayers were cotransfected with pRLuc31 and capsid donor plasmid in the presence of recombinant vaccinia virus v-TF7-3, expressing T7 RNA polymerase. Progeny viruses recovered from the transfection are labelled passage 1 (Pass 1) virus; progeny viruses recovered from subsequent infection of HeLa cells with passage 1 virus are labelled passage 2 (Pass 2) virus.
