. 1998 Oct;72(10):7972–7977. doi: 10.1128/jvi.72.10.7972-7977.1998

TABLE 1.

Relative efficiency of trans-encapsidation of poliovirus replicon by different viral capsids

Capsid used for trans-encapsidation^a	Virus recovered from transfection (PFU/ml [expt 1^b])	Relative efficiency of trans-encapsidation (%)^c
Capsid used for trans-encapsidation^a	Virus recovered from transfection (PFU/ml [expt 1^b])	Expt 1	Expt 2	Expt 3
Poliovirus	4 × 10⁸	100	100	100
Coxsackievirus B3	2 × 10⁸	6.1	3.7	n.d.
HRV14^d	1 × 10⁷	0.23	n.d.	1.2
Mengovirus	7 × 10⁷	0.06	n.d.	0.6
HAV	Negative	Negative	n.d.	n.d.

Viral capsids were produced following transfection with the corresponding viral cDNA constructs described in Materials and Methods.

Experiment 1 is the experiment represented in Fig. 7.

Relative efficiency was calculated by arbitrarily setting poliovirus trans-encapsidation at 100%, based on luciferase activity after infection. Values represent the percentage of luciferase activity resulting from infection of trans-encapsidated replicon. n.d., not done.

Plaque assay mixture for HRV14 was incubated at 34°C.