TABLE 2.
Histological assessment of the tissues among the biological replicates in 2 closely comparable cases (selected, based on the comparability of perioperative physiological parameters and trend of biochemical markers between the cases in both groups), in control (n = 2) and NEVP (n = 2) groups by H&E and TUNEL staining (grading: 0: none, 1: mild, 2: moderate, 3: severe; for ducts: 0: absent and 1: present)
| H&E | TUNEL | ||||||
|---|---|---|---|---|---|---|---|
| Group | Fat necrosis | Parenchyma | Hemorrhage | Duct integrity | Autolysis | Islets (× 4×) | Staining percent |
| Test 1 (Prep 60 min) | 0 | 1 | 0 | 1 | 1 | 1 | NA |
| Control 1 (Prep 60 min) | 1 | 2–3 | 1 | 1 | 1 | 2 | NA |
| Test 2 (Prep 60 min) | 1–2 | 3 | 1 | 0 | 1 | 1 | NA |
| Control 2 (Prep 60 min) | 1 | 1 | 0 | 1 | 1 | 3 | NA |
| Test 1 (Corpus nec) | 1 | 1 | 0 | 0 | 0 | 2 | 30%–60% |
| Control 1 (Corpus nec) | 1–2 | 3 | 2–3 | 0 | 2 | 0 | >60% |
| Test 2 (Corpus nec) | 1 | 1–2 | 1 | 1 | 0 | 2 | 30%–60% |
| Control 2 (Corpus nec) | 1–2 | 2–3 | 2 | 1 | 1 | 1 | 30%–60% |
| Test 1 (Duod nec) | NA | 1 | 1 | NA | 1 | NA | NA |
| Control 1 (Duod nec) | NA | 3 | 3 | NA | 3 | NA | NA |
| Test 2 (Duod nec) | NA | 0–1 | 1 | NA | 0 | NA | NA |
| Control 2 (Duod nec) | NA | 3 | 3 | NA | 3 | NA | NA |
Duod, duodenum; H&E, hematoxylin and eosin; Islet number, per high-power field; NA, not applicable; NEVP, normothermic ex vivo perfusion; Prep, postreperfusion; nec, necropsy; TUNEL, terminal deoxynucleotidyl transferase deoxynucleotide triphosphate nick end labeling.