TABLE 2.

Amino acid variation observed in human H3N2 viruses isolated from 1993 to 1997 in Japan^a

Protein	PB2 aa at position:									PB1 aa at position:											PA aa at position:
	82	107	221	318	539	553	559	570	697	14	56	83	97	163	179	216	382	439	517	622	212	250	256	275	276	312	341	342	349	350	376	409	432	437	548	557	712
Kit93	S	T	A	R	I	V	T	I	L	A	R	D	E	N	I	S	S	D	I	Q	R	S	K	D	C	R	V	L	E	T	P	N	V	Y	M	M	T
Aki94								M			K					G				R	L						I
Aki95								M		V	K					G				R													I	H	L		K
Toc95								M		V	K		D			G				R													I	H			K
Miy95				K				M			K		D			G				R			Q					M			Q		I				K
Shi95								M			K					G				R					S			M				I	I				K
Fuk96			T					M	I		K	N		S			N		V	R													I
Nii96								M	I		K			S	V				V	R													I
Fuk97	N	S				I	N	M	I		K					G				R		F				K				N						I
Shi97					L			M	I		K					G		G		R				H		K			K	N						I

Protein	HA (HA1 domain) aa at position:
	5	27	34	111	114	133	135	142	156	157	158	164	167	172	183	196	197	214	219	226	248	262	275	276	278	279
Kit93	G	K	I	I	D	D	G	G	K	S	E	L	S	G	H	V	Q	T	S	Q	T	N	G	T	S	F
Aki94		R					K			L			T				R	I	F					N		S
Aki95				T	G		K			L			T	D				I		I	I			N	N	S
Toc95				T	G		K			L			T	D				I		I			D	N	N	S
Miy95				T	G		K			L			T	D				I		I			D	N	N	S
Shi95				T	G		K			L			T	D				I		I			D	N	N	S
Fuk96	R		V	T	G		T			L			T	D				I		V		S		N	N	S
Nii96				T	G	N	T			L			T	D				I		V		S	D	N	N	S
Fuk97				N	S	N	T	R		L			T	D	L	A		I		V		S		K	N	S
Shi97				N	S	N	T	R	Q	L	k	Q	T	D				I		V		S		N	N	S

Protein	NP aa at position:										NA aa at position:
	18	65	127	197	239	259	290	375	384	451	24	43	47	51	52	54	65	71	82	127	136	141	151	212	221	248	262	285	336	338	339	342	344	346	369	381	385	400	418	435
Kit93	E	R	D	I	M	S	N	E	R	A	M	S	N	I	P	Q	I	T	V	G	Q	N	D	V	N	E	V	P	Y	Q	N	N	K	S	E	G	T	S	I	K
Aki94		K	E	V		L		G	G			N	S	M	L	E			A								I			R	K						K	R		E
Aki95							D					N		M	L	E			A								I			L			E	G	K		K	R	V	E
Toc95							D					N		M	L	E			A								I			L			E	G	K		K	R	V	E
Miy95							D			T	V	N		M	L	E			A		R					G	I			L			E	G	K		K	R		E
Shi95							D			T		N		M	L	E			A							G	I			L			E	G	K		K	R		E
Fuk96							D			T		N		M	L	E		I	A	D			G	A	K		I			H	L		E	G	K		K	R		E
Nii96							D			T		N		M	L	E			A	D					K	G	I	S	H	L			E	G	K		K	R		E
Fuk97	D	K	E	V	V	L	D	G	G			N		M	L	G			A	D		K			K	G	I		H	L		D	E	G	K	E	K	R		E
Shi97	D	K	E	V	V	L	D	G	G			N		M	L	E	M		A	D					K	G	I		H	L			E	G	K	E	K	R		E

Protein	M1 aa at position:			M2 aa at position:				NS1 aa at position:												NS2 aa at position:
	219	227	230	16	21	43	57	33	66	68	71	101	112	129	144	164	209	212	228	26
Kit93	I	A	K	G	D	L	H	L	E	I	E	N	E	M	L	S	N	P	S	E
Aki94										V	K					P
Aki95				E	G		Y	I			K	D				P
Toc95				E	G	F	Y				K		D			P
Miy95									K					I		P
Shi95														I		P				V
Fuk96															I	P
Nii96															I	P
Fuk97	V	T	R												I	P	D	S
Shi97	V	T	R				Q									P			P

^aPredicted amino acid (aa) differences in the proteins of human H3N2 viruses isolated from 1993 to 1997. Only locations where differences were observed are indicated. Differences located in the proposed antigenic sights and of the HA and NA proteins are underlined with a single line. Amino acid differences in the proposed receptor binding domain of the HA protein, as well as the functional domain of the NA protein, are double underlined.