Figure 1.

H2A.Z loss in muscle fibers leads to reduced lifespan and display physical changes. (A) TA muscle extracts from 12-month-old mice were immunoblotted with the indicated antibodies. (B) Kaplan–Meier survival curves of males and females CTL (n = 24) and H2A.Z mdKO (n = 23) mice. The cumulative survival rate was plotted against age in months. The Log-rank test was performed to compare the two mouse groups (***P < 0.0001). (C) Dorsal (upper panel), lateral (middle panel) and dissected tibialis anterior (TA) muscles (lower panel) photographs of 12-month-old mice reveal a thinner body, loss of hair quality, a kyphosis and muscle loss of H2A.Z mdKO mice compare to their CTL littermate. (D) Body weight in 12-old-month males (CTL n = 9; H2A.Z mdKO n = 10). Whole body fat mass (E) and lean mass (F) evaluated by TD-NMR (n = 12, *P < 0.05, Mann–Whitney test).