Figure 6.
H2A.Z loss leads to increase DNA damages. (A) Representative image of TUNEL (A488) and 8-OHdG (A647) staining in transversal sections of TA muscle. (B) Representative image of 53BP1 and γH2A.X staining (scale bar 10 μm). Each myonucleus is spotted with an arrow. (C, D) Quantification of the fluorescence intensity of each myonucleus on 500 μm2 of the 53BP1 (C) and γH2A.X staining (D). (Error bars are the SEM calculated from 4 mice per group, Mann–Whitney test ***P< 0.0001). (E) Representative Western blot analysis of total protein extract of 12-month-old TA muscle from CTL and H2A.Z mdKO mice immunoblotted with H2AX and γH2AX. H4 was used as loading control.