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. 2024 Jan 28;52(6):3031–3049. doi: 10.1093/nar/gkae020

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — H2A.Z is necessary for GFP-Ku80 recruitment at DNA lesions sites. (A) Scheme of the sequential steps to obtain immortalized myoblasts (MB) and differentiated myotubes (MT) expressing the GFP-Ku80 in presence or absence of both H2A.Z for Frap analysis. (B) Accumulation of GFP-Ku80 after laser irradiation in CTL or H2A.Z dKO nuclei of seven-day differentiated myotubes. The damaged area has been delimited by a circle area and was spotted with a white arrow for each time point (scale bar 5 μm). (C) Chromatin extract fraction of isolated muscle nuclei immunoblotted with the indicated antibodies.