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. 2024 Feb 26;52(6):3180–3198. doi: 10.1093/nar/gkae129

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Transposition frequency comparison between Tn7 and Tn6022. Percent transposition measured and compared by the mating-out assay using prototypic Tn7 (TnsABC + TnsD) and Tn6022 transposition proteins (TnsABC + TniQ + TnsF) in the presence of a conjugal plasmid harboring a region of glmS (Tn7 experiments) or a 600 bp region of comM (Tn6022 experiments). The percentage of transposition events was calculated by patching 200 transconjugants to determine the percentage that had the transposon genetic marker (KanR). All experiments were performed in triplicate with 0.02% arabinose induction and 0.1mM IPTG for 90 min. All experiments were performed in triplicate with error bars representing the standard error of the mean.