(A) Levels of cytokines secreted into the supernatant by No Act Tregs, TCR Tregs, CAR Tregs, No Act Teff, TCR Teff, and CAR Teff 48h post-activation. (B) Intracellular levels of IFNG produced by No Act Tregs, TCR Tregs, CAR Tregs, No Act Teff, TCR Teff, and CAR Teff 18h post-activation. Representative countour plots on the left and summary data on the right. (C) Intracellular levels of IL-2 produced by No Act Tregs, TCR Tregs, CAR Tregs, No Act Teff, TCR Teff, and CAR Teff 18h post-activation. Representative contour plots on the left and summary data on the right. Values represent technical replicates of representative experiments. Bars represent mean ± SD. One-way ANOVA test with Tukey’s multiple comparison correction. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, not significant.