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[Preprint]. 2024 Apr 1:2024.03.31.587467. [Version 1] doi: 10.1101/2024.03.31.587467

Figure 8. Lowering CAR affinity improves CAR Treg suppressive function.

Figure 8.

(A) Inhibition of CellTrace Far Red (CTFR) labeled CD4+ T responder cell (Tresp) proliferation by Tregs. (B) Inhibition of CTFR labeled CD8+ Tresp proliferation by Tregs. (C) Downregulation of CD80 surface expression in CD80-NALM6 cells (aAPC – artificial antigen presenting cells) by Tregs. Representative histograms on the left and summary data on the right. (D) Treg cytotoxicity towards target NALM6 cells at different effector to target (E:T) ratios. (E) WT and PRF1 KO CAR Treg cytotoxicity towards target NALM6 cells at different E:T ratios. Values represent technical replicates of representative experiments. Bars represent mean ± SD. One-way ANOVA test with Tukey’s multiple comparison correction. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, not significant.