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[Preprint]. 2024 Apr 2:2023.10.29.564634. [Version 2] doi: 10.1101/2023.10.29.564634

Figure 4. Comparing BenzoHTag and HaloTag7 in no-wash, live-cell labeling experiments.

Figure 4.

(a) Labeling with varied concentrations of Bz-1 for 10 minutes in non-transfected U-2 OS cells and cells transfected with H2B-BenzoHTag or H2B-HaloTag7 (transfection efficiency was roughly 20%, Fig. S9). (b) Confocal microscopy imaging of H2B-BenzoHTag-transfected U-2 OS cells treated with 10 nM Bz-1. Cells were co-stained with Hoescht to highlight the nucleus. (c) Increase of Bz-1 fluorescence over time when added to U-2 OS cells that were transiently transfected with H2B-BenzoHTag or H2B-HaloTag7. Fluorescence intensities of at least 10 transfected cells for each condition were measured over the course of five minutes with the average background fluorescence of 10 non-transfected cells subtracted. See Supporting Information for details.