[Preprint]. 2024 Apr 2:2023.10.29.564634. [Version 2] doi: 10.1101/2023.10.29.564634

Table 1.

Summary of HaloTag Variant Sequences.

Variant	Mutations	Second-order rate constant (x10³ M⁻¹s⁻¹)	Percent change in fluorescence relative to HaloTag7	Emission maximum (nm)
HaloTag7	-	1.5 ± 0.17	-	532
Variant 1	F144S \| L246F	9.3 ± 2.0	14	532
Variant 2	V245A	11.0 ± 0.9	8	536
Variant 3	N195S \| V245A \| E251K \| S291P	8.3 ± 1.0	11	536
Variant 4	L161W \| V245A	10.3 ± 1.4	8	536
Variant 5	I211V \| V245A	41.3 ± 1.6	7	536
Variant 6	F144L \| L221S \| V245A	33.4 ± 2.4	9	536
Variant 7	F144L \| L221S \| V245A \| L246F	15.6 ± 1.4	13	534
Variant 8	F144L \| I211V \| L221S \| V245A	68.8 ± 8.2	14	536
Variant 9	F144L \| I211V \| L221S \| V245A \| L246F	44.6 ± 4.1	15	532
Variant 10 (BenzoHTag)	F144L \| I211V \| V245A	95.4 ± 9.2	16	536