FIG. 1.
Construction of recombinant VZV with a deletion in ORF32. The VZV genome is 124,884 bp in length (line 1) and contains UL, unique short (US), terminal repeat (TR), and internal repeat (IR) DNA sequences (line 2). NotI and MstII restriction fragments used to generate parental VZV are shown (lines 3 and 4). Cosmid MstII B-32D (line 5) has a deletion beginning before the start codon and ending at codon 123 of ORF32. Two identical, independent clones of cosmids MstII B-32D, termed MstII B-32DA and MstII B-32DB, were used to construct VZV with ORF32 deleted.