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[Preprint]. 2024 Apr 3:2024.04.02.587832. [Version 1] doi: 10.1101/2024.04.02.587832

Figure 7. The OPTN/KIF5B/TRAK1 complex promotes striking ON regeneration after ONC.

Figure 7.

A, Left, confocal images of ON wholemounts after optical clearance showing maximum intensity projection of regenerating axons labeled with CTB-Alexa 555 at 14dpc. Scale bar, 250 μm. ***: crush site. Right, quantification of regenerating axons at different distances distal to the lesion site. n = 4–9. Data are presented as means ± s.e.m, *: p<0.05, **: p<0.01, ****: p<0.0001, two-way ANOVA with Dunnett’s multiple comparisons test, compared to PTEN KO group. B, Light-sheet fluorescent images (bottom view, sagittal view and coronal view) of regenerating axons in ONs, optic chiasm, and optic tract in PTEN KO mice with mOPTN+TRAK1+KIF5B overexpression at 4 weeks post ONC (4wpc). Regenerating axons were labeled with CTB-Alexa 555 and CTB-Alexa 647 in both eyes separately. Higher magnification images of framed regions (a-e, a’-e’) are shown to the right. Scale bar, 500μm. C, Models of OPTN physiological role in axonal mitochondria transport, dysfunctional OPTN∆C in jeopardizing axonal mitochondria distribution and inducing neurodegeneration, and neuroprotection and axon regeneration of OPTN-TRAK1-KIF5B by increasing axonal mitochondria delivery.