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[Preprint]. 2024 Apr 3:2024.04.03.587983. [Version 1] doi: 10.1101/2024.04.03.587983

Temporal Ablation of Primary Cilia Impairs Brainwave Patterns Implicated in Memory Formation

Matthew Strobel, Liyan Qiu, Aldebaran Hofer, Xuanmao Chen
PMCID: PMC11014598  PMID: 38617207

Abstract

The primary cilium is a hair-like organelle that hosts molecular machinery for various developmental and homeostatic signaling pathways. Its alteration can cause severe ciliopathies such as the Bardet-Biedl and Joubert syndromes, but is also linked to Alzheimer’s disease, clinical depression, and autism spectrum disorder. These afflictions are caused by disturbances in a variety of genes but a common phenotype amongst them is cognitive impairment. Cilia-mediated neural function has generally been examined in relation to these diseases or other developmental defects, but the role of cilia in brain function and memory consolidation is unknown. To elucidate the role of cilia in neural activity and cognitive function, we temporally ablated primary cilia in adult mice before performing electroencephalogram/electromyogram (EEG/EMG) recordings. We found that cilia deficient mice had altered sleep architecture, reduced EEG power, and attenuated phase-amplitude coupling, a process that underlies memory consolidation. These results highlight the growing significance of cilia, demonstrating that they are not only necessary in early neurodevelopment, but also regulate advanced neural functions in the adult brain.

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