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. 2024 Feb 29;134(8):e174051. doi: 10.1172/JCI174051

Figure 9. Conditional Hif1a deletion in granulocytes significantly attenuates the expression of glycolytic genes concomitant with increased proinflammatory profiles.

Figure 9

(A) CD45+ cells were recovered by FACS from Mrp8NullHif1afl/fl and Mrp8CreHif1afl/fl mice (n = 4 mice/group) at days 3 (n = 1,629 and 2,366 cells, respectively) and 14 (n = 2,725 and 1,991 cells, respectively) after S. aureus PJI for single-cell sequencing. The resultant data sets were integrated and clustered using a UMAP procedure. (B) Expression levels of core glycolytic genes in the most abundant granulocyte clusters (G1 and G2). (C) Pathway analysis using differentially expressed genes identified in Mrp8NullHif1afl/fl and Mrp8CreHif1afl/fl granulocyte clusters. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, correlation-weighted Kolmogorov-Smirnov with Benjamini-Hochberg correction.