Fig. 2. TBL1XR1::TP63 regulates the T- and B-cells in mice.

(A) Strategy for insertion of the CAG-loxP-Stop-LoxP-TBL1XR1::TP63-IRES-EGFP cassette into the mouse Rosa26 locus. (B, C) Representative flow activated cell sorting (FACS) plot (B) and quantification (C) showing TCRb staining of viable cells. (D, E) Representative FACS plot (D) and quantification (E) showing CD4/CD8 staining of TCRb+ T cells; (F, G) Representative FACS plot (F) and quantification (G) showing Treg (Foxp3 staining) of TCRb+ T cells; (H, I) Representative FACS plot (H) and quantification (I) showing B cells (CD19/B220 staining) of viable cells; (J, K) Representative FACS plot (J) and quantification (K) showing FoB and MZB (CD21/CD23 staining) of B220+ B cells; (L, M) Representative FACS plot (L) and quantification (M) showing GCB (CD38/CD95 staining) of B220+ B cells; (N, O) Representative FACS plot (N) and quantification (O) showing naïve B cell (CD27/IgD staining) of B220+ B cells; (P, Q) Representative FACS plot (P) and quantification (Q) showing PC (CD138/B220 staining) of viable cells. All spleen samples were from 11-week-old Cre and Cre/tgTT mice. Data are presented as mean ± SD, *P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 as compared between indicated groups (Student’s t-test). n=4 mice/arm except for B cells (n=6 mice for Cre and n=7 mice for Cre/tgTT). (R) Quantification of cytokines in peripheral blood serum from 11-week-old Cre and Cre/tgTT mice. Cytokine quantification is presented as the ratio in Cre/tgTT versus Cre mice (n=3 mice/arm).