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. 2024 Feb 9;130(8):1249–1260. doi: 10.1038/s41416-023-02550-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Scheme of study design. Created with BioRender.com, b Volcano plot of differential gene expression analysis according to primary and recurrent disease. For each gene, the log fold-change (log2FC) and the –log10 p-value are plotted. The dotted lines indicate the set cutoff values for further analysis (p < 0.05, |log2FC | > 0.8). Red dots mark the seven makers chosen for IHC.