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. 2024 Mar 15;25(4):1752–1772. doi: 10.1038/s44319-024-00097-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) Representative images for in vitro maturation of oocyte in control (2.5 mM), 5 mM, and 10 mM conditions. Images show the same oocyte in GV (germinal vesicle), MI (Metaphase I), and MII (Metaphase II) stage during maturation. (B) Number of counts (UMIs) for every cell for MII oocytes (n = 20 for 2.5 mM, n = 21 for 5 mM, n = 20 for 10 mM) (C) number of genes/features detected per cell for MII oocytes. (D) PCA plot of oocytes cultured in control (2.5 mM) and mildly diabetic (5 mM) conditions (n = 20 for 2.5 mM, n = 21 for 5 mM, n = 20 for 10 mM). (E) Volcano plot displaying no differentially expressed genes between oocytes undergoing IVM in control and mildly diabetic conditions. (F) Violin plots depicting oocyte-specific genes. (n = 17 control oocytes, n = 16 5 mM oocytes). (G) Violin plot depicting TET3 levels in human oocytes (n = 17 control oocytes, n = 16 5 mM oocytes). UMI, Unique Molecular Identifier. Data information: Data depicted here is in biological replicates. In panels (B) and (C), data was analyzed using one-way ANOVA with Bonferroni’s post hoc test. In panel e, data was analyzed using MAST. For boxplots in panels (F) and (G), the lower and upper hinges of the box depict the 1st and 3rd quartiles, and the middle line depicts the median. The upper and lower whiskers extend to the largest or smallest value no further than 1.5 times the interquartile range (1.5*IQR) from the hinge. Data beyond the end of the whiskers are plotted as a larger dot, which are then the minimum and/or maximum values depicted. If no large dots are present, the whiskers extend to the minimum and/or maximum values. *P < 0.05. Source data are available online for this figure.