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. 2024 Feb 27;25(4):1814–1834. doi: 10.1038/s44319-024-00098-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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We used smFISH, coupled to G3BP-IF, to further substantiate our findings using the control transcript GAPDH (A) and core SG transcripts AHNAK (B) and MKI67 (C). Transcript localization upon arsenite stress for either 0 min, 30 min or 1 h in WT and NAT10 KO HeLa cells is shown. Red is smFISH probe, green is G3BP and blue is DAPI. Scale bar, 10 µm. Quantification of the fraction of AHNAK mRNA (D) and MKI67 (E) in SG per cytoplasm in different conditions. 20 cells per each condition were counted, each circle represents a single cell. Data is represented as Mean ± SEM, unpaired two-tailed Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Source data are available online for this figure.