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. 2024 Apr 12;15:3194. doi: 10.1038/s41467-024-47401-6

Fig. 2. Epidermal, muscle and neuronal clusters in Pristina leidyi.

Fig. 2

A – UMAP visualisation highlighting Epidermis (blue), Muscle (red), and Neuron (yellow) clusters. B – In situ HCRs and expression plot of epidermis marker PrileiEVm008309t1, showing extensive signal in the epidermal cells across the body. The bottom left panel is a close-up of the top left panel. C – In situ HCR and expression plot of the neuronal marker PrileiEVm000558t1, showing groups of neuronal cell bodies across the worm’s body. The right microscopy panel is a close-up from the middle microscopy panel. D – In situ HCR and expression plot of the muscle marker PrileiEVm000300t1, showing expression along the worm. The middle microscopy panel is a close-up from the left panel, evidencing muscle fibres (arrowheads). Scale bars are 50 μm unless otherwise specified. All expression patterns displayed in the figure were observed in, at least, 3 different individuals.