Figure 5.

Images and drawings of phenotypes observed in long-term Notch-knockdown transgenic Hydra. The images labeled with a–e represent light-microscopy, while a’–e’ display Ds-Red fluorescence. (A) “Ectopic tentacles” phenotype: a was similar to the phenotypes observed in HvNICD-overexpressing transgenic Hydra, b–e showed thickening of different lengths at their bases (ectopic tentacles are indicated by white triangles, thickenings at the bases of ectopic tentacles are shown by green arrows). (B) “Two-headed” phenotype: ectopic heads initially located in the oral half of the body column (a–c), and moving into the foot region after three weeks (a’’, b’’). Ectopic heads are indicated by white arrows. (C) “Y-shaped” polyps with the joint positioned in the budding zone. (D) “Two feet” phenotypes with extra feet indicated by yellow arrows. Two gonads in Fig. 5A-c and Fig. 5C-a were indicated by a thin white arrow.