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. 2024 Apr 12;14:8553. doi: 10.1038/s41598-024-58837-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HyWnt3 and HyAlx FISH of regenerating polyps 72 h after decapitation. Stacks of laser-confocal microscopic images. (A) Expression of HyWnt3 in abnormally and normally regenerated HvNotch-knockdown polyps (a–f: non-regenerated head or single-tentacle head, g–i: normally regenerated head with enlargements g’–i’). Control polyps 1#, 2# and 3# displayed normal regeneration (j–l). (B) Expression of HyAlx in HvNotch-knockdown polyps of strain 11# with non-regenerated heads (a, b and enlargement b’), single-tentacle heads of strains 4#, 11# and 13# (c–e and enlargements c’–e’) and control polyps with normally regenerated heads of strains 1#, 2# and 3# (f–h).