FIG. 1.

Expression and localization of LRP during a productive infection of MDBK cells. MDBK cells were infected with BHV-1 (2 TCID₅₀/cell) for the indicated times (hours postinfection). Mock-infected cells were designated time 0. (A) Whole-cell lysates were prepared and Western blot analysis was performed with the P2 antibody as described previously (11, 24). (B) At 36 hpi, cells were scraped and washed two times with PBS. Cells were then incubated in hypotonic buffer for 10 min (lane 1), pelleted, resuspended in hypotonic buffer, and lysed by Dounce homogenization (lane 2). Nuclei were pelleted and resuspended in hypertonic buffer. After 30 min of incubation at 4°C, the nuclei were centrifuged and the nuclear extract was removed (lane 3). Nuclei were extracted two more times with hypertonic buffer for 30 min each time (lanes 4 and 5). The final nuclear pellet was lysed by boiling in SDS-PAGE buffer (lane 6). Each lane contains extract from approximately 100,000 cells. Sizes are indicated in kilodaltons.