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Exposure of embryonic zebrafish to cell culture medium and nanomaterials. Embryonic zebrafish were staged and dechorionated at 4 h post-fertilization (hpf) and placed in a 96 well plate at 6 hpf. The plates contained various test media and/or the ENMs were sonicated prior to being added to the plate. A total of 12 mortality and morphological effects were examined at 24 and 120 hpf. Figure created with BioRender.com.
